Biolegend, 354504, PE anti-human CD304 (Neuropilin-1) Antibody, 100tests

CD304, also known as neuropilin-1, BDCA-4 and VEGF165R, is a 140 kD type I transmembrane protein. Its extracellular region contains 2 CUB, 2 FV/FVIII, and one MAM domain; a soluble isoform is produced by alternative mRNA splicing. CD304 is involved in angiogenesis, neural development, and tumor metastasis. It's expressed by plasmacytoid dendritic cells, thymocytes, neurons, endothelium, and a subset of T FH cells. CD304 is also expressed in several carcinomas, and a high expression of this molecule in prostate cancer correlates with a poor prognosis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CD304-Fc Fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed Tandon S, et al. 2022. Br J Haematol. 196:e34. PubMed Combes AJ, et al. 2022. Cell. 185:184. PubMed Kitai H, et al. 2022. BMC Biol. 20:248. PubMed Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed Congy-Jolivet N, et al. 2022. EBioMedicine. 80:104047. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID: AB_11219200 (BioLegend Cat. No. 354503) AB_11219194 (BioLegend Cat. No. 354504)
Structure: Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution: Plasmacytoid dendritic cells, thymocytes, subset of follicular helper T cells (TFH), endothelial cells, neurons, some carcinomas
Function: Angiogenesis, neuronal development, tumor metastasis
Ligand/Receptor: VEGF165, semaphorin-3A
Cell Type: Dendritic cells, Endothelial cells, Neurons, Tfh, Thymocytes
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Mizui M and Kikutani H. 2008. Immunity 28:302. 2. Hamerlik P, et al. 2012. J. Exp. Med. 209:507. 3. Karjalainen K, et al. 2011. Blood 117:920. 4. Lepelletier Y, et al. 2007. P. Natl. Acad. Sci. USA 104:5545.
Gene ID: 8829
UniProt: View information about CD304 on UniProt.org
Clone: 12C2
Regulatory Status: RUO
Other Names: BDCA-4
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.