Biolegend, 354506, APC anti-human CD304 (Neuropilin-1) Antibody, 100tests

CD304, also known as neuropilin-1, BDCA-4 and VEGF165R, is a 140 kD type I transmembrane protein. Its extracellular region contains 2 CUB, 2 FV/FVIII, and one MAM domain; a soluble isoform is produced by alternative mRNA splicing. CD304 is involved in angiogenesis, neural development, and tumor metastasis. It's expressed by plasmacytoid dendritic cells, thymocytes, neurons, endothelium, and a subset of T FH cells. CD304 is also expressed in several carcinomas, and a high expression of this molecule in prostate cancer correlates with a poor prognosis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CD304-Fc Fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Sugrue JA, et al. 2022. Cell Rep Med. 3:100804. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed Bencze D, et al. 2022. Int J Mol Sci. 23:. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID: AB_11219393 (BioLegend Cat. No. 354505) AB_11219600 (BioLegend Cat. No. 354506)
Structure: Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution: Plasmacytoid dendritic cells, thymocytes, subset of follicular helper T cells (TFH), endothelial cells, neurons, some carcinomas
Function: Angiogenesis, neuronal development, tumor metastasis
Ligand/Receptor: VEGF165, semaphorin-3A
Cell Type: Dendritic cells, Endothelial cells, Neurons, Tfh, Thymocytes
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Mizui M and Kikutani H. 2008. Immunity 28:302. 2. Hamerlik P, et al. 2012. J. Exp. Med. 209:507. 3. Karjalainen K, et al. 2011. Blood 117:920. 4. Lepelletier Y, et al. 2007. P. Natl. Acad. Sci. USA 104:5545.
Gene ID: 8829
UniProt: View information about CD304 on UniProt.org
Clone: 12C2
Regulatory Status: RUO
Other Names: BDCA-4
Isotype: Mouse IgG2a, κ