Biolegend, 354607, Brilliant Violet 421™ anti-human CD290 (TLR10) Antibody, 25tests

CD290, also known as TLR10, is a 91-100 kD glycosylated protein belonging to the toll-like receptor family. It is expressed on B cell, Treg cells, and plasmacytoid dendritic cells but absent on T cells, particularly non-Treg and granulocytes. It can interact with MyD88 and plays an important role in innate immunity and pattern recognition.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified extracellular domain of human TLR10 (amino acids 20-474) (NP_001017388 )
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Guan Y, et al. 2010. J. Immunol. 184:5094.
RRID: AB_3662263 (BioLegend Cat. No. 354607) AB_3662263 (BioLegend Cat. No. 354608)
Structure: Member of the toll-like receptor family, type I membrane protein, 91-100 kD
Distribution: B cells, Treg cells, plasmacytoid DCs
Function: Interacts with MyD88 and TRAF6, which results in NF-kB activation and cytokine secretion
Ligand/Receptor: MyD88 and TRAF6
Cell Type: B cells, Dendritic cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Chuang T, et al. 2001. Biochim. Biophys. Acta. 1518:157. 2. Hornung V, et al. 2002. J. Immunol. 168:4531. 3. Bourke E, et al. 2003. Blood 102:956.
Gene ID: 81793
UniProt: View information about CD290 on UniProt.org
Clone: 3C10C5
Regulatory Status: RUO
Other Names: Toll like receptor 10
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.