Biolegend, 354714, PE/Dazzle™ 594 anti-human CD301 (CLEC10A) Antibody, 100tests

CD301, also known as CLEC10A, macrophage galactose/N-acetylgalactosamine (GalNAc) specific lectin (MGL), DCASGPR, and HML, is a 40 kD type II transmembrane glycoprotein, which belongs to the C-type lectin family. Human CD301 consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region.  CD301 is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages. The expression level is upregulated by immunosuppressant dexamethasone. Human CD301 has an exclusive specificity for rare terminal GalNAc structures, which are revealed on the tumor-associated mucin MUC1 and CD45 (RA, RB, and RC but not RO isoforms). This interaction inhibits effector T cell activation and induces their apoptosis. CD301 also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant CD301 (extracellular domain)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: H037G3 antibody binding is divalent cation dependent.
RRID: AB_3683377 (BioLegend Cat. No. 354713) AB_3683377 (BioLegend Cat. No. 354714)
Structure: 40 kD type II transmembrane glycoprotein, consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region
Distribution: Immature myeloid dendritic cells, activated macrophages
Function: Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, immune response
Interaction: Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
Ligand/Receptor: Glycans containing terminal GalNAc moieties
Cell Type: Dendritic cells, Macrophages
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Van Vliet SJ, et al. 2006. Nat. Immunol. 7:1200. 2. Higashi N, et al. 2002. J. Biol. Chem. 277:20686. 3. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892. 4. Valladeau J, et al. 2001. J. Immunol. 167:5767. 5. Suzuki N, et al. 1996. J. Immunol. 156:128.
Gene ID: 10462
UniProt: View information about CD301 on UniProt.org
Clone: H037G3
Regulatory Status: RUO
Other Names: Macrophage galactose/Nacetylgalactosamine (GalNAc) specific lectin, MGL, DCASGPR, HML CLECSF13, CLECSF14, HML2
Isotype: Mouse IgG2a, κ