Biolegend, 354908, PerCP/Cyanine5.5 anti-human CD21 Antibody, 100tests

CD21 is a 145 kD transmembrane protein also known as complement C3d receptor (C3dR), complement receptor 2 (CR2), and Epstein-Barr virus receptor. CD21 is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells. CD21 is the receptor used by Epstein-Barr virus to infect B cells and is also the complement receptor for C3d. CD21 has also been shown to interact with a number of proteins, including CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1).
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4.
Application References(PubMed link indicates BioLegend citation): Björck P, et al. 1993. Eur. J. Immunol. 23:1771. Frémeaux-Bacchi V, et al. 1996. Eur. J. Immunol. 26:1497. Ling NR, et al. 1995. Clin. Exp. Immunol. 101:369. Wang, C, et al. 2011. BMC Immunol. 12:53. (IHC)
Product Citations: Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed Wentink M, et al. 2015. Clin Immunol. Available online 30 August 2015. PubMed Zumaquero E, et al. 2019. Elife. 8. PubMed Johnson P, et al. 2015. Clin Cancer Res. 21:1321. PubMed Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
RRID: AB_2561543 (BioLegend Cat. No. 354907) AB_2561544 (BioLegend Cat. No. 354908)
Structure: Transmembrane protein, multiple SUSHI domains, approximate molecular weight 145 kD
Distribution: Expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, some epithelial cells
Function: Epstein-Barr virus receptor on B cells used for infection
Interaction: CD23, CD19, complement component 3, annexin VI, CD81, iC3b, complement receptor 1, interferon alpha 1
Ligand/Receptor: C3d, EBV
Cell Type: B cells, Dendritic cells, Epithelial cells, T cells, Thymocytes
Biology Area: Cell Biology, Complement, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Kishimoto T, Eds. 1997. Leukocyte Typing VI. Garland Publishing Inc. 2. Moore MD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9194. 3. Szakonyi G, et al. 2001. Science 292:1725. 4. Weis JJ, et al. 1984. Proc. Natl. Acad. Sci. USA 81:881.
Gene ID: 1380
UniProt: View information about CD21 on UniProt.org
Clone: Bu32
Regulatory Status: RUO
Workshop: V CD21.4, VI CD21.5
Other Names: Complement C3d receptor (C3dR), complement receptor 2 (CR2), Epstein-Barr virus receptor
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.