Biolegend, 355201, Purified anti-human SSEA-5 Antibody, 50μg

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that contains the motif Fuc1-2Galβ1-3GlcNAcβ, which is characteristic of H-1 antigens. SSEA-5 is expressed on undifferentiated embryonic stem cells and pluripotent stem cells. It is developmentally regulated, and its expression is lost with cell differentiation.
50μg
Verified Reactivity: Human
Reported Reactivity: African Green
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human embryonic stem cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1 and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Tang C, et al. 2011. Nat Biotechnol. 29:829. (IHC, IP)
Product Citations: Takahashi K, et al. 2021. PLoS Genet. 17:e1009587. PubMed
RRID: AB_2561465 (BioLegend Cat. No. 355201) AB_2561466 (BioLegend Cat. No. 355202)
Structure: Glycan, H type-1 backbone, 127, and >190 kD
Distribution: Embryonic stem cells, pluripotent stem cells
Cell Type: Embryonic Stem Cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Andrews PW. 2011. Nat. Biotechnol. 29:803. 2. Itskovitz-Eldor J. 2011. Cell. Stem. Cell. 9:291.
Gene ID: NA
Clone: 8e11
Regulatory Status: RUO
Other Names: Stage-Specific Embryonic Antigen-5
Isotype: Mouse IgG1, κ