Biolegend, 355208, FITC anti-human SSEA-5 Antibody, 100tests

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that contains the motif Fuc1-2Galβ1-3GlcNAcβ, which is characteristic of H-1 antigens. SSEA-5 is expressed on undifferentiated embryonic stem cells and pluripotent stem cells. It is developmentally regulated, and its expression is lost with cell differentiation.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human embryonic stem cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1 and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Tang C, et al. 2011. Nat Biotechnol. 29:829. (IHC, IP)
RRID: AB_2561827 (BioLegend Cat. No. 355208)
Structure: Glycan, H type-1 backbone, 127, and >190 kD
Distribution: Embryonic stem cells, pluripotent stem cells
Cell Type: Embryonic Stem Cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Andrews PW. 2011. Nat. Biotechnol. 29:803. 2. Itskovitz-Eldor J. 2011. Cell. Stem. Cell. 9:291.
Gene ID: NA
Clone: 8e11
Regulatory Status: RUO
Other Names: Stage-Specific Embryonic Antigen-5
Isotype: Mouse IgG1, κ