Biolegend, 355305, PE anti-human CD367 (CLEC4A) Antibody, 25tests

CD367 (CLEC4A), also known as lectin-like immunoreceptor (LLIR) and dendritic cell immunoreceptor (DCIR), is a type II transmembrane protein belonging to C-type lectin domain family, and was previously designated as CLECSF6. It contains one carbohydrate recognition domain in its C-terminal extracellular domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain.  CLEC4A is mainly expressed on monocytes, macrophages, dendritic cells, neutrophils, and B cells. It is also found on the surface of CD4 + T cells in rheumatoid arthritis (RA) patients. CLEC4A has been reported to function as a receptor for HIV, cross-present and cross-prime human CD8 + T cells. It might also function as a negative player by recruiting the SHP-1 or/and SHP-2 phosphotase through its immunoreceptor tyrosine-based inhibitory motif (ITIM).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ectodomain of CLEC4A-human Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
RRID: AB_2561626 (BioLegend Cat. No. 355306)
Structure: Type II transmembrane protein with single C-type lectin domain, contain immunoreceptor tyrosine-based inhibitory motif (ITIM)
Distribution: Neturophils, monocytes, dendritic cells and B cells
Function: Receptor for HIV, cross-presentation and cross-priming of human CD8+ T cells, inhibit intracellular signaling
Cell Type: Neutrophils, Monocytes, Dendritic cells, B cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Costimulatory Molecules
Molecular Family: CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Lambert AA, et al. 2011. Blood 117:6589. 2. Klechevsky E, et al. 2010. Blood 116:1685. 3. Kanazawa N, et al. 2002. J. Invest Dermatol. 118:261. 4. Voehringer D, et al. 2001. J. Immunol. 167:4838.
Gene ID: 50856
UniProt: View information about CLEC4A on UniProt.org
Clone: 9E8
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: DCIR, LLIR, CLECSF6, CD367
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.