Biolegend, 355605, PE/Cyanine7 anti-human CD227 (MUC-1) Antibody, 25tests

Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1/SOS1/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2/neu.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Jurkat cells transfected with MUC1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This clone shows stronger binding to the glycosylated compared to the non-glycosylated peptide1.
Application References(PubMed link indicates BioLegend citation): Song W, et al. 2012. Int. J Oncol. 41:1977. PubMed
Product Citations: Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed
RRID: AB_2564194 (BioLegend Cat. No. 355605) AB_2564195 (BioLegend Cat. No. 355606)
Structure: 500-1000 kD proteoglycan (glycosylated form)
Distribution: Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers
Interaction: Complexes with SOS1 and Grb2 in RAS signaling
Ligand/Receptor: ICAM-1 and Her2/neu (ErbB2)
Cell Type: Dendritic cells, Epithelial cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Gendler SJ. 2001. J. Mammary Gland Biol. Neoplasia. 6:339. 2. Agrawal B, et al. 1998. Cancer Res. 58:4079. 3. Rahn JJ, et al. 2004. J. Biol. Chem. 279:29386.
Gene ID: 4582
UniProt: View information about CD227 on UniProt.org
Clone: 16A
Regulatory Status: RUO
Other Names: Mucin-1, MUC-1, Episialin, HMFG antigen, MAM6
Isotype: Mouse IgG1, λ