Biolegend, 356901, Purified anti-human CD185 (CXCR5) Antibody, 25μg

CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR5-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Law JC, et al. 2021. J Immunol. 206:37. PubMed Spoerl S, et al. 2021. Eur J Immunol. 51:1436. PubMed Lu X, et al. 2021. J Exp Med. 218: . PubMed Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed Lartey S, et al. 2020. J Infect Dis. 221:21. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed de Jong SE, et al. 2021. Nature Immunology. 22(5):654-665. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Schulten V, et al. 2018. J Allergy Clin Immunol. 141:775. PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Majri SS, et al. 2018. J Immunol. 200:110. PubMed
RRID: AB_2561810 (BioLegend Cat. No. 356901) AB_2561811 (BioLegend Cat. No. 356902)
Structure: G-protein coupled receptor, 7 transmembrane regions, 42 kD
Distribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
Function: Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Ligand/Receptor: CXCL13
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241. 2. León B, et al. 2012. Nat. Immunol. 13:681. 3. Crotty S. 2011. Annu. Rev. Immunol. 29:621. 4. Kerfoot SM, et al. 2011. Immunity 34:947. 5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.
Gene ID: 643
UniProt: View information about CD185 on UniProt.org
Clone: J252D4
Regulatory Status: RUO
Other Names: BLR1, MDR15
Isotype: Mouse IgG1, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.