Biolegend, 356920, Brilliant Violet 421™ anti-human CD185 (CXCR5) Antibody, 100tests

CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR5-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Sannier G, et al. 2023. Cell Rep Med. 4:100955. PubMed Hagel J, et al. 2021. J Immunol. 206:3073. PubMed Nayrac M, et al. 2022. Cell Rep. 39:111013. PubMed Nicolas A, et al. 2023. iScience. 26:105904. PubMed Goyal G, et al. 2022. Adv Sci (Weinh). 9:e2103241. PubMed Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed Acharya A, et al. 2020. J Virol. 95:e01657. PubMed Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed Yang L, et al. 2020. Genes Dis. 7:128. PubMed Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Maehara T, et al. 2018. Life Sci Alliance. 1:e201800050. PubMed Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Tauzin A, et al. 2021. Cell Host Microbe. . PubMed Vanoni G, et al. 2021. eLife. 10:00. PubMed Paris R, et al. 2015. PLoS One. 10: 0144767. PubMed Högelin KA, et al. 2021. iScience. 24:103078. PubMed Gamradt S, et al. 2021. iScience. 24:103312. PubMed
RRID: AB_2562302 (BioLegend Cat. No. 356919) AB_2562303 (BioLegend Cat. No. 356920)
Structure: G-protein coupled receptor, 7 transmembrane regions, 42 kD
Distribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
Function: Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Ligand/Receptor: CXCL13
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241. 2. León B, et al. 2012. Nat. Immunol. 13:681. 3. Crotty S. 2011. Annu. Rev. Immunol. 29:621. 4. Kerfoot SM, et al. 2011. Immunity 34:947. 5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.
Gene ID: 643
UniProt: View information about CD185 on UniProt.org
Clone: J252D4
Regulatory Status: RUO
Other Names: BLR1, MDR15
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.