Biolegend, 357104, FITC anti-human CD77 Antibody, 100tests

CD77 is also known as Gb3 glycosphingolipid. It was found specifically expressed on Burkitt's lymphoma cells and on a subset of germinal center B lymphocytes. CD77 has been described as a receptor for Shiga Toxin and Verotoxin 1 (VT1), which is produced by Shigella dysenteriae and some strains of E. coli . It is used as a marker for germinal center stage B-cells. CD19 has been reported as a ligand for CD77 and their interaction functions in adhesion and signal transduction at a specific stage of B cell development. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human peripheral blood mononuclear cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for relevant formats) include: Western blotting1 and immunohistochemical staining of frozen tissue sections1.
Application References(PubMed link indicates BioLegend citation): Fyfe G, et al. 1987. J. Immunol. 139:2187. (WB, IHC)
Product Citations: Maldonado-Báez L, et al. 2013. J Cell Biol. 233:201. PubMed Mohamed LA, et al. 2019. Exp Neurol. 27:316. PubMed Roodsant TJ, et al. 2024. iScience. 109178:27. PubMed Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
RRID: AB_2562160 (BioLegend Cat. No. 357103) AB_2562161 (BioLegend Cat. No. 357104)
Structure: Glycosphingolipid
Distribution: Burkitt's lymphoma cells, a subset of germinal center B lymphocytes
Function: Involved in cell adhesion and signaling transduction by binding CD19
Ligand/Receptor: Receptor of Shiga Toxin and Verotoxin 1 (VT1) and CD19
Cell Type: B cells, Lymphocytes
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Tétaud C, 2003. J. Biol. Chem. 278:45200. 2. Maloney MD, 1994. J. Exp. Med. 1994 180:191. 3. Mangeney M, et al. 1991. Eur. J. Immunol. 21:1131. 4. Mangeney M, et al. 1993. Cancer Res. 53:5314.
Gene ID: NA
Clone: 5B5
Regulatory Status: RUO
Workshop: VI CD77.5
Other Names: globotriaosylcermamide (Gb3), Burkitt's lymphoma associated antigen (BLA), cermadie trihexoside (CTH), and Pk blood group antigen
Isotype: Mouse IgM, κ