Biolegend, 357409, PE/Cyanine7 anti-human CD4 Antibody, 25tests

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CD4 T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Hu Z, et al. 2022. Nat Metab. 4:759. PubMed Ma T, et al. 2022. Elife. 11: . PubMed Giannou AD, et al. 2023. Immunity. 56:125. PubMed Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed Wang K, et al. 2020. Signal Transduct Target Ther. 0.404861111. PubMed Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed Minns D, et al. 2021. Front Immunol. 12:633486. PubMed Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed Mu Z, et al. 2021. Genome Biol. 122:22. PubMed Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed Su X, et al. 2022. J Transl Med. 20:378. PubMed Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed Egedal JH, et al. 2021. Mucosal Immunol. 14:1203. PubMed Łyszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed Corrado M, et al. 2020. Cell Metab. 32:981. PubMed Pekayvaz K, et al. 2022. Nat Commun. 13:1018. PubMed
RRID: AB_2565661 (BioLegend Cat. No. 357409) AB_2565662 (BioLegend Cat. No. 357410)
Structure: 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution: Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells
Interaction: Part of the TCR/CD3 complex
Ligand/Receptor: β2 domain from the MHC class II
Cell Type: T cells, Monocytes, Macrophages, NKT cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: A161A1
Regulatory Status: RUO
Other Names: T4/Leu-3
Isotype: Rat IgG2b, κ
Q: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
A: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.