Biolegend, 357413, PerCP/Cyanine5.5 anti-human CD4 Antibody, 25tests

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CD4 T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Kim S, et al. 2023. Front Immunol. 14:1062365. PubMed Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed Woan KV, et al. 2021. Cell Stem Cell. 28:2062. PubMed Chen Y, et al. 2022. Sci Immunol. 7:eabp8328. PubMed Idiz UO, et al. 2022. J Med Virol. 94:4744. PubMed Cannons JL, et al. 2021. Cell Rep. 37:109804. PubMed Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed Yang W, et al. 2019. Nat Med. 25:767. PubMed Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed Chen Y, et al. 2020. Cell. 1496:183. PubMed Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed Karlsson J, et al. 2020. Nat Commun. 1.773611111. PubMed
RRID: AB_2565665 (BioLegend Cat. No. 357413) AB_2565666 (BioLegend Cat. No. 357414)
Structure: 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution: Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells
Interaction: Part of the TCR/CD3 complex
Ligand/Receptor: β2 domain from the MHC class II
Cell Type: T cells, Monocytes, Macrophages, NKT cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: A161A1
Regulatory Status: RUO
Other Names: T4/Leu-3
Isotype: Rat IgG2b, κ
Q: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
A: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.