Biolegend, 357509, PerCP/Cyanine5.5 anti-human CD269 (BCMA) Antibody, 25tests

CD269, also known as B cell maturation antigen (BCMA), is a 27 kD, single pass transmembrane protein with one TNFR-Cys repeat on its extracellular domain. CD269 is a B cell maturation factor, essential for the long-term survival of plasma cells. It is expressed by plasmablasts, plasma cells, and germinal center B cells. The ligands of CD269 are BAFF and APRIL, and its cytoplasmic domain binds several of the TRAF family members.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BCMA-mouse IgG Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Meng L, et al. 2021. Front Oncol. 11:691705. PubMed Domenger A, et al. 2022. EMBO Mol Med. 14:e14740. PubMed Cordero H, et al. 2021. Nat Commun. 12:5761. PubMed
RRID: AB_2566528 (BioLegend Cat. No. 357509) AB_2566529 (BioLegend Cat. No. 357510)
Structure: Single pass transmembrane protein, member of the TNFR superfamily, 1 TNFR-Cys domain, 27 kD
Distribution: Plasmablasts, plasma cells, germinal center B cells
Function: B cell maturation factor, essential for survival of plasma cells
Interaction: Associates with several TRAF family members
Ligand/Receptor: BAFF, APRIL
Cell Type: B cells, Plasma cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Coquery CM and Erickson LD. 2012. Crit. Rev. Immunol. 32:287. 2. Notas G, et al. 2012. J. Immunol. 189:4748. 3. Rickert RC, et al. 2011. Immunol. Rev. 244:115. 4. Mesin L, et al. 2011. J. Immunol. 187:2867.
Gene ID: 608
UniProt: View information about CD269 on UniProt.org
Clone: 19F2
Regulatory Status: RUO
Other Names: BCM, TNFRSF17
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.