Biolegend, 357520, Brilliant Violet 421™ anti-human CD269 (BCMA) Antibody, 100tests

CD269, also known as B cell maturation antigen (BCMA), is a 27 kD, single pass transmembrane protein with one TNFR-Cys repeat on its extracellular domain. CD269 is a B cell maturation factor, essential for the long-term survival of plasma cells. It is expressed by plasmablasts, plasma cells, and germinal center B cells. The ligands of CD269 are BAFF and APRIL, and its cytoplasmic domain binds several of the TRAF family members.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BCMA-mouse IgG Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Duan M, et al. 2023. Cell Rep. 112682:42. PubMed Kadow S, et al. 2022. Cancers (Basel). 14:. PubMed
RRID: AB_2687357 (BioLegend Cat. No. 357519) AB_2687358 (BioLegend Cat. No. 357520)
Structure: Single pass transmembrane protein, member of the TNFR superfamily, 1 TNFR-Cys domain, 27 kD
Distribution: Plasmablasts, plasma cells, germinal center B cells
Function: B cell maturation factor, essential for survival of plasma cells
Interaction: Associates with several TRAF family members
Ligand/Receptor: BAFF, APRIL
Cell Type: B cells, Plasma cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Coquery CM and Erickson LD. 2012. Crit. Rev. Immunol. 32:287. 2. Notas G, et al. 2012. J. Immunol. 189:4748. 3. Rickert RC, et al. 2011. Immunol. Rev. 244:115. 4. Mesin L, et al. 2011. J. Immunol. 187:2867.
Gene ID: 608
UniProt: View information about CD269 on UniProt.org
Clone: 19F2
Regulatory Status: RUO
Other Names: BCM, TNFRSF17
Isotype: Mouse IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.