Biolegend, 357817, Brilliant Violet 421™ anti-human ROR1 Antibody, 25tests

ROR1, also known as NTRKR1, is a type I transmembrane protein and member of the ROR subfamily of surface receptors. ROR1 consists of one frizzled domain, one Ig-like C2-type domain, one kringle domain, and one kinase domain with no catalytic activity. ROR1 is expressed on embryonic tissue, in the central nervous system and on some cancer cells, and is used as a marker for B-cell chronic lymphocytic leukemia. Wnt5a has been identified as a ligand for ROR1.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone 2A2 binds to the membrane distal Ig-domain of ROR1. Clone 2A2 has also been shown to exhibit cross reactivity towards mouse ROR12.
Application References(PubMed link indicates BioLegend citation): Dave H, et al. 2012. PLoS One 7:e52655. (FC) Baskar S, et al. 2012. MAbs. 4:349. (FC)
RRID: AB_2716118 (BioLegend Cat. No. 357817) AB_2716119 (BioLegend Cat. No. 357818)
Structure: Type I transmembrane protein, consist of 1 frizzled domain, 1 Ig-like C2-type domain, 1 kringle domain, and 1 non-functional kinase domain; member of the ROR subfamily of surface receptors
Distribution: Embryonic tissue, central nervous system, chronic lymphocytic leukemia cells
Ligand/Receptor: Wnt5a
Cell Type: Embryonic Stem Cells, Leukemia
Biology Area: Immunology, Stem Cells
Antigen References: Bicocca VT, et al. 2012. Cancer Cell. 22:656. Zhang S, et al. 2012. PLoS One 7:e31127. Uhrmacher S, et al. 2011. Leuk Res. 35:1360. Yang J, et al. 2011. PLoS One 6:e21018. Grumolato L, et al. 2010. Genes Dev. 24:2517.
Gene ID: 4919
UniProt: View information about ROR1 on UniProt.org
Clone: 2A2
Regulatory Status: RUO
Other Names: Receptor tyrosine kinase-like orphan receptor 1, Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase receptor-related 1, NTRKR1
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.