Biolegend, 357821, Alexa Fluor® 647 anti-human ROR1 Antibody, 25tests

ROR1, also known as NTRKR1, is a type I transmembrane protein and member of the ROR subfamily of surface receptors. ROR1 consists of one frizzled domain, one Ig-like C2-type domain, one kringle domain, and one kinase domain with no catalytic activity. ROR1 is expressed on embryonic tissue, in the central nervous system and on some cancer cells, and is used as a marker for B-cell chronic lymphocytic leukemia. Wnt5a has been identified as a ligand for ROR1.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 2A2 binds to the membrane distal Ig-domain of ROR1. Clone 2A2 has also been shown to exhibit cross reactivity towards mouse ROR12.
Application References(PubMed link indicates BioLegend citation): Dave H, et al. 2012. PLoS One 7:e52655. (FC) Baskar S, et al. 2012. MAbs. 4:349. (FC)
RRID: AB_2734384 (BioLegend Cat. No. 357821) AB_2734385 (BioLegend Cat. No. 357822)
Structure: Type I transmembrane protein, consist of 1 frizzled domain, 1 Ig-like C2-type domain, 1 kringle domain, and 1 non-functional kinase domain; member of the ROR subfamily of surface receptors
Distribution: Embryonic tissue, central nervous system, chronic lymphocytic leukemia cells
Ligand/Receptor: Wnt5a
Cell Type: Embryonic Stem Cells, Leukemia
Biology Area: Immunology, Stem Cells
Antigen References: Bicocca VT, et al. 2012. Cancer Cell. 22:656. Zhang S, et al. 2012. PLoS One 7:e31127. Uhrmacher S, et al. 2011. Leuk Res. 35:1360. Yang J, et al. 2011. PLoS One 6:e21018. Grumolato L, et al. 2010. Genes Dev. 24:2517.
Gene ID: 4919
UniProt: View information about ROR1 on UniProt.org
Clone: 2A2
Regulatory Status: RUO
Other Names: Receptor tyrosine kinase-like orphan receptor 1, Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase receptor-related 1, NTRKR1
Isotype: Mouse IgG1, κ