Biolegend, 358205, PE/Cyanine7 anti-human GPR56 Antibody, 25tests

GPR56, also known as TM7XN1, is an orphan G-protein-coupled receptor (GPCR) containing seven transmembrane domains and a mucin-like domain in the N-terminal region. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons and encodes 693 amino acids.  GPR56 is expressed in a wide range of tissues, such as brain, thyroid gland, and heart. Mutations in human GPR56 gene were found to cause a specific brain malformation called bilateral frontoparietal polymicrogyria. Recently, GPR56 was found specifically expressed on cytotoxic NK and T lymphocytes, including CD8+, CD4+, and γδ T cells, which might affect the migratory properties of these cells. GPR56 was also found significantly downregulated in some cancer cells, suggesting that it may play a role in growth suppression and cancer metastasis.
25tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human GPR56-mouse Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone CG4 cross-reacts with mouse1.
Application References(PubMed link indicates BioLegend citation): Rao TN, et al. 2015. Stem Cell Res. 14:307. (FC) PubMed
Product Citations: Wanjalla CN, et al. 2021. Cell Reports Medicine. 2(2):100205. PubMed Wanjalla CN, et al. 2019. Front Immunol. 10:408. PubMed Tomellini E, et al. 2020. Cell Reports. 28(4):1063-1073.e5.. PubMed Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed
RRID: AB_2562089 (BioLegend Cat. No. 358205) AB_2562090 (BioLegend Cat. No. 358206)
Structure: Contains seven transmembrane domains and a mucin-like domain in the N-terminal region
Distribution: T cells, NK cells, wide range of tissues such as brain, thyroid gland, and heart
Function: May be involved in immune cell migration, cancer cell growth metastasis, and neuron development
Cell Type: NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Peng YM, et al. 2011. J. Leukoc. Biol. 90:735. 2. Shashidhar S, et al. 2005. Oncogene 24:1673. 3. Piao X, et al. 2004. Science 303:2033. 4. Liu M, et al. 1999. Genomics 55:296.
Gene ID: 9289
UniProt: View information about GPR56 on UniProt.org
Clone: CG4
Regulatory Status: RUO
Other Names: TM7XN1
Isotype: Mouse IgG1, κ