Biolegend, 358412, PE/Cyanine7 anti-human CD120b Antibody, 100tests

CD120b, also known as TNFRII or TNFRSF1B, belongs to the TNF receptor superfamily and can interact with ligands TNF-α and TNF-β (also known as LT-α). It is a 75 kD type I transmembrane protein expressed on lymphocytes, monocytes, and granulocytes. When present in a heterocomplex with CD120a, it induces the recruitment of anti-apoptotic molecules, c-IAP1 and c-IAP2 that possess E3 ubiquitin ligase activity. Mutations in CD120b are associated with TNF-associated periodic syndrome (TRAPS) or periodic fever syndrome.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial CD120b recombinant protein expressed in 293E cells (22-211aa).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Volpin V, et al. 2020. Cancer Immunol Res. 8:1163. PubMed
RRID: AB_2564395 (BioLegend Cat. No. 358411) AB_2564396 (BioLegend Cat. No. 358412)
Structure: 75 kD, type I transmembrane protein, member of the TNF-receptor superfamily, forms a heterocomplex with TNFRI
Distribution: Expressed on cells of hematopoietic lineage including monocytes, lymphocytes, and granulocytes
Function: When complexed with TNFRI, recruits anti-apoptotic molecules c-IAP1 and c-IAP2 that possess ubiquitin ligase activity; cysteine rich extracellular region
Interaction: Interacts with TTRAP and TRAF2
Ligand/Receptor: TNF-α and TNF-β (LT-α)
Cell Type: Granulocytes, Lymphocytes, Monocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Transcription Factors
Molecular Family: CD Molecules
Antigen References: 1. Andrews JS, et al. 1990. J. Immunol. 144:2582. 2. Nophar Y, et al. 1990. EMBO J. 9:3269. 3. Kohno T, et al. 1990. Proc. Natl. Acad. Sci. USA 87:8331. 4. Tartaglia LA, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9292. 5. Hijdra D, et al. 2012. J. Inflamm. (Lond). 9:38. 6. Chen X, et al. 2013. J. Immunol. 190:1076.
Gene ID: 7133
UniProt: View information about CD120b on UniProt.org
Clone: 3G7A02
Regulatory Status: RUO
Other Names: TNFRSF1B, p75, p75TNFR, TNFR2, TNFR80, TBPII
Isotype: Rat IgG2a, κ