Biolegend, 358510, PE/Dazzle™ 594 anti-human/mouse Bcl-6 Antibody, 100tests

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to T FH . This molecule is also expressed in some B cell lymphomas and breast cancer cells.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Sequence 226-398 of murine BCL6 fused with GST
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting1,2,3. NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Application References(PubMed link indicates BioLegend citation): Vikstrom, et al. 2010. Science 330:1095. (WB) Kallies A, et al. 2011. Blood 117:1869. (WB) Lüthje K, et al. 2012. Nat. Immunol. 13:491. (WB) Tonti E, et al. 2012. J. Immunol. 188:3217. (ICFC)
RRID: AB_2566193 (BioLegend Cat. No. 358509) AB_2566194 (BioLegend Cat. No. 358510)
Structure: Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution: Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells
Function: Transcription factor, master regulator of germinal center reaction
Interaction: BLIMP-1
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Basso K and Dalla-Favera R. 2012. Immunol. Rev. 247:172. 2. Vinuesa CG, and Cyster JG, 2011. Immunity 35:671. 3. Kitano M, et al. 2011. Immunity 34:961. 4. Baumjohann D, et al. 2011. J. Immunol. 187:2089. 5. Johnston RJ, et al. 2009. Science. 325:1006. 6. Tran TH, et al. 2010. Cancer Res. 70:1711.
Gene ID: 604
UniProt: View information about Bcl-6 on UniProt.org
Clone: 7D1
Regulatory Status: RUO
Other Names: B-cell lymphoma 6, BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype: Rat IgG2a, κ