Biolegend, 358602, Purified anti-human LOX-1 Antibody, 100μg

Lectin-like oxidized LDL receptor 1 (LOX-1) also known as CLEC8A is a 31 kD single pass type II transmembrane protein and member of the C-type lectin superfamily. LOX-1 is a scavenger receptor and is involved in endocytosis, phagocytosis and cytokine production. CLEC8A is expressed by endothelial cells, smooth muscle cells, platelets, fibroblasts, macrophages, and is upregulated by inflammatory and oxidative stimuli. LOX-1 recognizes numerous ligands including lipoproteins, apoptotic cells heat shock proteins, C-reactive protein and oxidized LDL. LOX-1 is implicated in atherosclerosis and vascular inflammation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: LOX-1 ectodomain: Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescent staining of acetone-fixed frozen human skin sections1.
Application References(PubMed link indicates BioLegend citation): Li D, et al. 2012. J. Exp. Med. 209:109. (IHC)
RRID: AB_2562272 (BioLegend Cat. No. 358602)
Structure: Single pass, type II, transmembrane protein, 31 kD, homodimer, member of the C-type lectin superfamily.
Distribution: Endothelial cells, smooth muscle, platelets, fibroblasts, macrophages; Upregulated by inflammatory and oxidative stimuli
Function: Scavenger receptor, involved in endocytosis, phagocytosis and cytokine production
Ligand/Receptor: Recognizes numerous ligands including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, oxidized LDL
Cell Type: Dendritic cells, Endothelial cells, Fibroblasts, Macrophages, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules
Antigen References: 1. Yin Y, et al. 2013. Eur. J. Cell Biol. 92:150. 2. Khaidakov M. and Mehta JL. 2012. PLoS One. 7:e46973. 3. Sawamura T, et al. 2012. Curr. Opin. Lipidol. 23:439. 4. Lin FY, et al. 2011. J. Immunol. 186:4405. 5. Parlato S, et al. 2010. Blood 115:1554.
Gene ID: 4973
UniProt: View information about LOX-1 on UniProt.org
Clone: 15C4
Regulatory Status: RUO
Other Names: OLR1, LOX1, LOXIN, SLOX1, CLEC8A, SCARE1
Isotype: Mouse IgG2a, κ