Biolegend, 358609, Brilliant Violet 421™ anti-human LOX-1 Antibody, 25tests

Lectin-like oxidized LDL receptor 1 (LOX-1) also known as CLEC8A is a 31 kD single pass type II transmembrane protein and member of the C-type lectin superfamily. LOX-1 is a scavenger receptor and is involved in endocytosis, phagocytosis and cytokine production. CLEC8A is expressed by endothelial cells, smooth muscle cells, platelets, fibroblasts, macrophages, and is upregulated by inflammatory and oxidative stimuli. LOX-1 recognizes numerous ligands including lipoproteins, apoptotic cells heat shock proteins, C-reactive protein and oxidized LDL. LOX-1 is implicated in atherosclerosis and vascular inflammation.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: LOX-1 ectodomain: Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescent staining of acetone-fixed frozen human skin sections1.
Application References(PubMed link indicates BioLegend citation): Li D, et al. 2012. J. Exp. Med. 209:109. (IHC)
Product Citations: Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed Koh J, et al. 2020. Eur J Immunol. 50:1810. PubMed
RRID: AB_2728342 (BioLegend Cat. No. 358609) AB_2728343 (BioLegend Cat. No. 358610)
Structure: Single pass, type II, transmembrane protein, 31 kD, homodimer, member of the C-type lectin superfamily.
Distribution: Endothelial cells, smooth muscle, platelets, fibroblasts, macrophages; Upregulated by inflammatory and oxidative stimuli
Function: Scavenger receptor, involved in endocytosis, phagocytosis and cytokine production
Ligand/Receptor: Recognizes numerous ligands including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, oxidized LDL
Cell Type: Endothelial cells, Platelets, Fibroblasts, Macrophages, Dendritic cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules
Antigen References: 1. Yin Y, et al. 2013. Eur. J. Cell Biol. 92:150. 2. Khaidakov M. and Mehta JL. 2012. PLoS One. 7:e46973. 3. Sawamura T, et al. 2012. Curr. Opin. Lipidol. 23:439. 4. Lin FY, et al. 2011. J. Immunol. 186:4405. 5. Parlato S, et al. 2010. Blood 115:1554.
Gene ID: 4973
UniProt: View information about LOX-1 on UniProt.org
Clone: 15C4
Regulatory Status: RUO
Other Names: OLR1, LOX1, LOXIN, SLOX1, CLEC8A, SCARE1
Isotype: Mouse IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.