Biolegend, 359414, Brilliant Violet 421™ anti-human CD194 (CCR4) Antibody, 100tests

CD194, also known as CCR4, is a CC chemokine receptor. It binds CCL17 and CCL22 and is expressed on a subset of T and B cells, basophils, monocytes, and NK cells. Human Th2 cells are characterized by the expression of CCR4 and CCR8, and these receptors are regulated differently during Th2 development. Human peripheral blood Tregs can be divided into two distinct populations based on the expression of CCR4. Freshly isolated Tregs express CCR4 and presumably represent memory-type Tregs, and CCR4 - Tregs require CD3-mediated activation to acquire a regulatory activity. Depletion of CCR4 + T cells leads to Th1-type polarization of CD4 + T cells and augmentation of CD8 + T cell responses to tumor antigens. CCR4 and its ligands are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CCR4 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
RRID: AB_2562434 (BioLegend Cat. No. 359413) AB_2562435 (BioLegend Cat. No. 359414)
Structure: GPCR, seven transmembrane receptor
Distribution: Expressed on a subset of T and B cells, basophils, monocytes, NK cells
Function: Migration of inflammatory and regulatory cells to the target tissues
Ligand/Receptor: CCL17 and CCL22
Cell Type: B cells, Basophils, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Katschke KJ, et al. 2001 Arthritis Rheum. 44:1022. 2. Colantonio L, et al. 2002 Eur. J. Immunol. 32:1264. 3. Jakubzick C et al. 2004 Am. J. Pathol. 165:1211. 4. Morimoto Y, et al. 2005 J. Leukoc. Biol. 78:753. 5. Baatar J, et al. 2007 J. Immunol. 178:4891. 6. Kusumoto M, et al. 2007 J. Interferon. Cytokine Res. 27:901.
Gene ID: 1233
UniProt: View information about CD194 on UniProt.org
Clone: L291H4
Regulatory Status: RUO
Other Names: CKR4, K5-5, CMKBR4, ChemR13, CC-CKR-4, MGC88293, HGCN:14099
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.