Biolegend, 359812, PE/Cyanine7 anti-human CD41/CD61 Antibody, 100tests

CD41/CD61, also known as gpIIb/IIIa, is a member of a family integrin receptors. This is a complex comprised by CD41 and CD61 through non-covalent association. CD41/CD61 is mainly expressed by platelets and megakaryocytes. The resting form of the CD41/CD61 complex is involved in platelet activation and aggregation by binding to immobilized fibrinogen. After activation, CD41/CD61 becomes a receptor for soluble fibrinogen and several other RGD-containing adhesive proteins such as von Willebrand Factor (vWF) and fibronectin. An absence or dysfunction of CD41/CD61 on the platelet surface results in an inherited bleeding disorder, called Glanzmann Thromsasthenia (GT). CD41/CD61 has been found on murine hematopoietic progenitor cells, indicating that this complex may play a role in regulating hematopoietic development.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human platelets
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking (aggregation)2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 359804). A2A9/6 is recognized as having high affinity to gpIIb/IIIa. It has been shown to inhibit platelet aggregation induced by a variety of agonists and the initiation of clot formation.
Application References(PubMed link indicates BioLegend citation): Basani RB, et al. 1996. Blood. 88:167. (IP) Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. (Block)
Product Citations: Scala S, et al. 2023. Nat Commun. 14:3068. PubMed
RRID: AB_2564346 (BioLegend Cat. No. 359811) AB_2564347 (BioLegend Cat. No. 359812)
Structure: Complex of CD41 and CD61 through non-covalent association.
Distribution: Platelets and megakaryocytes.
Function: Mediates attachment to cells of diverse matrix proteins.
Ligand/Receptor: Fibrinogen, RGD-containing adhesive proteins such as vonWillebrand Factor (vWF) and fibronectin.
Cell Type: Megakaryocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Matsumura-Takeda K, et al. 2007. Stem Cell. 25:862. 2. Corbel C, et al. 2005. 49:279. 3. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. 4. Clemetson KJ, et al. 1994. Curr. Opin. Hematol. 1:388.
Gene ID: 36903674
UniProt: View information about CD41 or CD61 on UniProt.org
Clone: A2A9/6
Regulatory Status: RUO
Other Names: gpIIb/gpIIIa
Isotype: Mouse IgG2a, κ