Biolegend, 359912, PE/Cyanine7 anti-human CD309 (VEGFR2) Antibody, 100tests

CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human KDR recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Steimle JD, et al. 2023. Cell Rep. 112665:42. PubMed Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed Gruber MM, et al. 2021. Histochem Cell Biol. 156:363. PubMed Dong Y, et al. 2020. J Leukoc Biol. 108:1711. PubMed Zhou F, et al. 2018. Cancer Manag Res. 0.727083333. PubMed
RRID: AB_2563551 (BioLegend Cat. No. 359911) AB_2563552 (BioLegend Cat. No. 359912)
Structure: Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family
Distribution: Endothelial cells, embryonic tissues
Ligand/Receptor: VEGF-A, VEGF-C, and VEGF-D splice isoforms
Cell Type: Endothelial cells
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication. 2. Ferrara N and Gerber HP. 2002. Acta. Haematol. 106:148. 3. Murohara T, et al. 1998. Circulation. 97:99.
Gene ID: 3791
UniProt: View information about CD309 on UniProt.org
Clone: 7D4-6
Regulatory Status: RUO
Other Names: KDR, Vascular Endothelial Receptor 2 (VEGF-R2, VEGFR2, VEGFR)
Isotype: Mouse IgG1, κ