Biolegend, 360604, PE anti-human CD198 (CCR8) Antibody, 100tests

CCR8, also known as ChemR1, TER1, and CKR-L1, belongs to the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. CCR8 is expressed on some subsets of T cells, monocytes, macrophages, eosinophils, thymocytes, Langerhans cells, neurons, and vascular smooth muscle cells. Binding with its ligand CCL1, CCR8 plays a role in the regulation of monocyte chemotaxis and thymic cell apoptosis. It has been reported that CCR8 is also a receptor for CCL18. CCR8 is also believed to be an alternative coreceptor to CD4 for HIV-1 infection.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CCR8-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed Sanada S, et al. 2019. Kidney Int Rep. 4:447. PubMed Zeng W, et al. 2022. Oncogene. Online ahead of print. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Liu L, et al. 2021. Biochem Pharmacol. 188:114565. PubMed
RRID: AB_2562614 (BioLegend Cat. No. 360603) AB_2562615 (BioLegend Cat. No. 360604)
Structure: Seven hydrophobic transmembrane domains
Distribution: Vascular smooth muscle cells, macrophages, eosinophils, peritoneal macrophages, thymocytes, T cells, Langerhans cells and neurons
Function: Regulation of monocyte chemotaxis and thymic cell apoptosis
Ligand/Receptor: CCL1, CCL18
Cell Type: Dendritic cells, Eosinophils, Langerhans cells, Macrophages, Neurons, T cells, Thymocytes
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Tiffany HL, et al. 1997. J. Exp. Med. 186:165. 2. Goya I, et al. 1998. J. Immunol. 160:1975. 3. Lee S, et al. 2000. J. Virol. 74:6946. 4. Chensue SW, et al. 2001. J. Exp. Med. 193:573. 5. Qu C, et al. 2004. J. Exp. Med. 200:1231. 6. Isolam SA, et al. 2003. J. Exp. Med. 210:1889.
Gene ID: 1237
UniProt: View information about CD198 on UniProt.org
Clone: L263G8
Regulatory Status: RUO
Other Names: CC-CKR-8,C-C CKR-8, CCR-8, CC chemokine receptor 8, CDw198, CDw198 antigen, CD198, chemokine (C-C motif) receptor 8, chemokine (C-C) receptor 8, TER1, ChemR1, and CKR-L1
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.