Biolegend, 360606, Biotin anti-human CD198 (CCR8) Antibody, 100μg

CCR8, also known as ChemR1, TER1, and CKR-L1, belongs to the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. CCR8 is expressed on some subsets of T cells, monocytes, macrophages, eosinophils, thymocytes, Langerhans cells, neurons, and vascular smooth muscle cells. Binding with its ligand CCL1, CCR8 plays a role in the regulation of monocyte chemotaxis and thymic cell apoptosis. It has been reported that CCR8 is also a receptor for CCL18. CCR8 is also believed to be an alternative coreceptor to CD4 for HIV-1 infection.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CCR8-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2629656 (BioLegend Cat. No. 360606)
Structure: Seven hydrophobic transmembrane domains
Distribution: Vascular smooth muscle cells, macrophages, eosinophils, peritoneal macrophages, thymocytes, T cells, Langerhans cells and neurons
Function: Regulation of monocyte chemotaxis and thymic cell apoptosis
Ligand/Receptor: CCL1, CCL18
Cell Type: Dendritic cells, Eosinophils, Langerhans cells, Macrophages, Neurons, T cells, Thymocytes
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Tiffany HL, et al. 1997. J. Exp. Med. 186:165. 2. Goya I, et al. 1998. J. Immunol. 160:1975. 3. Lee S, et al. 2000. J. Virol. 74:6946. 4. Chensue SW, et al. 2001. J. Exp. Med. 193:573. 5. Qu C, et al. 2004. J. Exp. Med. 200:1231. 6. Isolam SA, et al. 2003. J. Exp. Med. 210:1889.
Gene ID: 1237
UniProt: View information about CD198 on UniProt.org
Clone: L263G8
Regulatory Status: RUO
Other Names: CC-CKR-8,C-C CKR-8, CCR-8, CC chemokine receptor 8, CDw198, CDw198 antigen, CD198, chemokine (C-C motif) receptor 8, chemokine (C-C) receptor 8, TER1, ChemR1, and CKR-L1
Isotype: Mouse IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.