Biolegend, 360713, Alexa Fluor® 647 anti-human CD16 Antibody, 25tests

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NK cell-enriched fraction from human peripheral blood.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G84. Donor variability has been observed for clone B73.1 staining1, especially on granulocytes.
Application References(PubMed link indicates BioLegend citation): Perussia B, et al. 1983. J. Immunol. 130:2133. Lanier LL, et al. 1985. J. Exp. Med. 162:2089. Perussia B, et al. 1984. J. Immunol. 133:180. Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope)
RRID: AB_2563020 (BioLegend Cat. No. 360713) AB_2563021 (BioLegend Cat. No. 360714)
Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor: IgG Fc receptor III (FcγRIII)
Cell Type: Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711. 2. Palmer BE, et al. 2005. J. Immunol. 175:8415. 3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183. 4. Wood KL, et al. 2005. Clin. Immunol. 117:294. 5. Björkström NK, et al. 2008. J. Immunol. 181:4219.
Gene ID: 22142215
UniProt: View information about CD16 on UniProt.org
Clone: B73.1
Regulatory Status: RUO
Other Names: FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3, CD16a
Isotype: Mouse IgG1, κ