Biolegend, 360802, Purified anti-human CXCL16 Antibody, 100μg

CXCL16, a 27 kD chemokine, exists in membrane-bound and soluble forms.  Unlike classic chemokines, CXCL16 possesses a mucin-like stalk, a transmembrane domain, and a cytoplasmic tail with a tyrosine phosphorylation domain, allowing it to be expressed on the cell surface.  Structurally, it is similar to fractalkine.  It is expressed by dendritic cells, monocytes, and T cells.  T cells can also migrate in response to CXCL16. CXCL16 can also act as a scavenger receptor on macrophages. Its receptor is CXCR6/Bonzo.  It can bind oxidized LDL and thus may play an important role in atherosclerosis.  Recent studies have shown a role for CXCL16 in kidney fibrosis and in autoimmunity.  CXCL16 is also a co-receptor for HIV.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human SR-PSOX-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats of this clone) include: in vitro blocking of chemotactic activity of CXCR6 expressing cells1. Clone 22-19-12 recognizes the chemokine domain of CXCL16 and has been shown to inhibit chemotactic activity to CXCR6-expressing cells by soluble CXCL16.1
Application References(PubMed link indicates BioLegend citation): Shimaoka T, et al. 2003. J. Immunol. 171:1647. (FC, Block)
RRID: AB_2562794 (BioLegend Cat. No. 360802)
Structure: ~27 kD, exists as a membrane bound and soluble forms
Distribution: Produced by dendritic cells and T cells
Function: Binds to oxidized LDL and may play an important role in atherosclerosis
Interaction: Interacts with CXCR6/Bonzo
Ligand/Receptor: CXCR6
Cell Type: Dendritic cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Matloubian M, et al. 2000. Nat. Immunol. 1:298. 2. Wilbanks A, et al. 2001. J. Immunol. 166:5145. 3. Shimaoka T, et al. 2003. J. Immuniol. 171:1647. 4. Chandrasekar B, et al. 2004. J. Biol. Chem. 179:3188. 5. Shimaoka T, et al. 2004. J. Leukoc. Biol. 75:267.
Gene ID: 58191
UniProt: View information about CXCL16 on UniProt.org
Clone: 22-19-12
Regulatory Status: RUO
Other Names: SRPSOX, CXCXLG16, SR-PSOX
Isotype: Mouse IgG1, κ