Biolegend, 360903, PE anti-human IL-27/IL-35 EBI3 (monomer, dimer, heterodimer) Antibody, 25tests

Epstein-Barr virus induced gene-3, also known as EBI3, is an IL-12 p40-related protein that can heterodimerize with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 diferentiation, increase cytolytic potential of CD8+ T cells, and impact B cell differentiation and Ig production.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human IL-27
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Alenazy MF, et al. 2021. Sci Rep. 11:5629. PubMed Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed Wang K, et al. 2018. Medicine (Baltimore). 97:e0710. PubMed
RRID: AB_2562879 (BioLegend Cat. No. 360903) AB_2562880 (BioLegend Cat. No. 360904)
Structure: Hematopoietin receptor family, subunit of IL-27 and IL-35
Distribution: B cells, monocytes and dendritic cells
Function: Pro- and anti-inflammatory
Interaction: IL-27 p28 subunit, IL-35 p35 subunit
Cell Type: B cells, Dendritic cells, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Pflanz S, et al. 2002. Immunity. 16: 779. 2. Takeda A, et al. 2003. J. immunol. 170: 4886. 3. Pflanz S, et al. 2004. J. Immunol. 172: 2225. 4. Li MF, et al. 2013. Dev. Comp. Immunol. 41: 514.
Gene ID: 10148
UniProt: View information about IL-27 IL-35 on UniProt.org
Clone: B032F6
Regulatory Status: RUO
Other Names: Epstein Barr virus induced gene 3, IL-27B, IL-35 subunit
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.