Biolegend, 361401, Biotin anti-human IL-34 Antibody, 50μg

Human IL-34 shares a sequence identity of 99.6%, 72% and 71% with chimpanzee, rat, and mouse IL-34, respectively, on the amino acid level. The IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif, and does not share sequence homology with M-CSF; nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines are differing in their ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests differential domain binding of the receptor to IL-34 and CSF and as a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-34
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5 - 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human IL-34 recombinant protein ranging from 5000 to 78 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection: The biotinylated E033E8 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified E0320E7 antibody (Cat. No. 361301) as the capture antibody and recombinant human IL-34 (Cat. No. 577909) as the standard.Note: For testing human IL-34 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human IL-34 ELISA Kit with Pre-coated Plates (Cat. No. 439607 & 439608) are specially developed and recommended.
Product Citations: Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed Han N, et al. 2021. STAR Protocols. 2(2):100460. PubMed
RRID: AB_2563034 (BioLegend Cat. No. 361401) AB_2563035 (BioLegend Cat. No. 361402)
Structure: Secreted glycoprotein, homodimer
Distribution: IL-34 mRNA is expressed in many different tissues including: spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon
Function: IL-34 increases monocyte viability, induces macrophage proliferation, and synergizes with other cytokines to generate macrophages and osteoclasts from cultured progenitors. IL-34 promotes the formation of the colony forming unit-macrophage (CFU-M) in human bone marrow culture. IL-34 supports RANKL-induced osteoclastogenesis by promoting the adhesion and proliferation of osteoclast progenitors. Human IL-34 promotes osteoclast differentiation from peripheral blood mononucleated cells. In addition, systematic administration of IL-34 to mouse increases the number of CD11b+ cells and reduces bone mass. IL-1ß and TNF-α induce the expression of interleukin-34 mRNA in osteoblasts. IL-34 is also induced in macrophages infected with equine infectious anemia virus (EIAV).
Interaction: Macrophages, monocytes, myeloid cells
Ligand/Receptor: CSF-1R
Biology Area: Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Clanchy FI, et al. 2006. J. Leuko. Biol. 79:757. 2. Lin H, et al. 2008. Science 320:807. 3. Chihara T, et al. 2010. Cell. Death Differ. 17:1917. 4. Wei S, et al. 2010. J. Leuko. Biol. 88:495. 5. Eda H, et al. 2011. Rheumatol. Int. 31:1525.
Gene ID: 146433
UniProt: View information about IL-34 on UniProt.org
Clone: E033B8
Regulatory Status: RUO
Other Names: Interleukin 34, C16orf77
Isotype: Mouse IgG2a, λ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.