Biolegend, 361502, Purified anti-human HLA-DQ Antibody, 100μg

HLA-DQ is heterodimeric cell surface glycoprotein comprised of a 27 kD α (heavy) chain and a 32 kD β (light) chain. In contrast to other MHC class II molecules, both chains of HLA-DQ are polymorphic and the α chain shows a high degree of polymorphism. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In hematopoietic development, HLA-DR and DP are expressed first, followed by HLA-DQ. Variations in the HLA gene expression are crucial to graft survival.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Tü169 has been reported to react with HLA-DQ1 and HLA-DQ2. It also shows weak reactivity to HLA-DQ3.Additional reported applications (for the relevant formats) include: immunofluorescence2 and in vitro blocking1. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Benagiano M, et al. 2012. Proc. Natl. Acad. Sci. USA 109:1222. (Block) Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF) Nasi A, et al. 2013. J. Immunol. 191:3090. (FC) Turrini R, et al. 2011. Cancer Immunol. Immunother. 60:1639. (FC)
Product Citations: Chang SH, et al. 2019. Exp Mol Med. 51:28. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID: AB_2563137 (BioLegend Cat. No. 361502)
Structure: Ig superfamily, MHC class II, heterodimeric transmembrane protein
Distribution: B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Thorsby E. 1974. Transplant. Rev. 18:51. 2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207. 3. Servenius B, et al. 1984. EMBO J. 3:3209. 4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105. 5. Strassmann G, et al. 1985. Hum. Immunol. 13:125. 6. Trowsdale J, et al. 1985. Immunol. Rev. 85:5. 7. Dorman J, et al. 2000. Epidemiologic Reviews 22:218. 8. van Lith M, et al. 2010. J. Biol. Chem. 285:40800.
Gene ID: 31173119
UniProt: View information about HLA-DQ on UniProt.org
Clone: Tü169
Regulatory Status: RUO
Other Names: MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA
Isotype: Mouse IgG2a, κ