Biolegend, 361903, FITC anti-human CD45 Antibody, 25tests

CD45 is a 180-220 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Fry T, et al. 2003. Blood 101:2294. Mabaera R, et al. 2008. Blood 111:411. Knapp W, et al. 1989. Leukocyte Typing IV. New York: Oxford University Press.
RRID: AB_3097444 (BioLegend Cat. No. 361903) AB_3097444 (BioLegend Cat. No. 361904)
Structure: Tyrosine phosphatases, type I transmembrane protein, 180-220 kD
Distribution: Hematopoietic cells, not circulating erythrocytes or platelets
Function: TCR and BCR mediated activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788
UniProt: View information about CD45 on UniProt.org
Clone: Tü116
Regulatory Status: RUO
Workshop: IV N832
Other Names: LCA, T200
Isotype: Mouse IgG1, κ