Biolegend, 362545, FITC anti-human CD56 (NCAM) Antibody, 25tests

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myotube cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Walsh FS, et al. 1981. Nature 289:60. (FC) Pavlath GK, et al. 1986. J. Cell Biol. 102:124. (FC) Pavlath GK, et al. 1989. Nature 337:570. (FC) Pulido R, et al. 1988. J. Immunol. 140:3851. (FC)
Product Citations: Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed Poran A, et al. 2020. Cell Reports Medicine. 1(8):100141. PubMed Ott PA, et al. 2020. Cell. 183(2):347-362.e24. PubMed Camberos V, et al. 2019. Int J Mol Sci. 2.7375. PubMed Hebbar N, et al. 2022. Nat Commun. 13:587. PubMed Wu X, et al. 2021. Front Immunol. 12:731767. PubMed Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed Dong G, et al. 2020. Haematologica. . PubMed Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed Deng W, et al. 2021. Emerg Microbes Infect. 10:1272. PubMed
RRID: AB_2565963 (BioLegend Cat. No. 362545) AB_2565964 (BioLegend Cat. No. 362546)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparan sulfate
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: 5.1H11
Regulatory Status: RUO
Other Names: Leu-19, NKH1, NCAM-1
Isotype: Mouse IgG1, κ