Biolegend, 362574, Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF Antibody, 1mg

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronalderived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate. Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF Antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody contains ultra-low levels of endotoxin (<0.01 EU/μg of protein), is filtered through a 0.1 μm membrane, and is tested negative for mycoplasma and microbial growths. Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF Antibody is for research and further ex vivo cell processing use only. BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include: - Ultra-Low endotoxin level (<0.01 EU/μg) - Manufactured under serum-free conditions - Bulks tested negative for mycoplasma and bacterial/fungal growth - Serum-free hybridomas are negative for the presence of murine virus - Batch-to-batch consistency - Vendor qualification - Raw material traceability and documentation - Documented procedures and employee training - Equipment maintenance and monitoring records - Lot-specific certificates of analysis - QA review of released products - Quality audits per ISO 13485:2016
1mg
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myotube cells
Formulation: 0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Endotoxin Level: < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: Serum-Free hybridoma derived Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody purified by affinity chromatography
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF Antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.1 μm filtered) is recommended for highly sensitive assays. The antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody is intended for flow cytometry and for research or further ex vivo cell processing use only.
Application References(PubMed link indicates BioLegend citation): Walsh FS, et al. 1981. Nature. 289:60. (FC) Pavlath GK, et al. 1986. J. Cell Biol. 102:124. (FC) Pavlath GK, et al. 1989. Nature. 337:570. (FC) Pulido R, et al. 1988. J. Immunol. 140:3851. (FC)
Disclaimer: BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL, and myeloid leukemias
Function: adhesion
Ligand/Receptor: Heparan sulfate
Antigen References: Lanier L, et al. 1991. J. Immunol. 146:4421 Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71 Cremer H, et al. 1994. Nature. 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: 5.1H11
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.