Biolegend, 362630, Pacific Blue™ anti-His Tag Antibody, 100tests

This antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.
100tests
Verified Reactivity: Human, Mouse, Rat, All species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated His-tag peptide.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC. Clone J095G46 has been verified for immunocytochemistry (ICC).
RRID: AB_2904387 (BioLegend Cat. No. 362630)
Structure: 6 repeat His amino acid
Function: Epitope tag
Biology Area: Cell Biology, Immunology
Antigen References: Zhao C, et al. 2010. Anal Biochem. 399:237 Salichs E, et al. 2009. PLoS Genet. 5:e1000397 Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523 Lindner, P, et al. 1997. Biotechniques. 22:140 Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Gene ID: NA
UniProt: View information about His Tag on UniProt.org
Clone: J095G46
Regulatory Status: RUO
Other Names: Hexa histidine-tag, 6xHis-tag, 6XHis, 6His
Isotype: Mouse IgG2a, κ