Biolegend, 362702, Purified anti-human/mouse CD3ε (activation epitope) Antibody, 500μg

The CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex.  This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α/β or γ/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.
500μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified denatured CD3 proteins from human thymus
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested WB, IHC-P - Verified in vivo cell tracking - Reported in the literature, not verified in house ICC, IHC, IP - Reported by the developer, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For Western blotting, the suggested use of this reagent is 1.0 - 4.0 µg per mL. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone APA1/1 recognizes an activation-dependent intracellular epitope of the CD3ε chain. Additional reported applications (for the relevant formats of this clone) include: immunofluorescent staining of paraformaldehyde fixed sections1, in vivo tracking of T cells 2, and Western blotting3. The LEAF purified format is recommended for in vivo studies.
Application References(PubMed link indicates BioLegend citation): Salmerón A, et al. 1991. J. Immunol. 147:3047. (ICFC, IF) Risueño RM, et al. 2005. Blood. 106:601. (IF, in vivo tracking, Mouse Reactivity) Risueño RM, et al. 2008. PLos One. 3:e1747. (WB) Gil D, et al. 2005. J. Exp. Med. 201:517. Risueño RM, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:9625.
Product Citations: Srivastava R, et al. 2023. Antib Ther. 253:6. PubMed Kuai Y, et al. 2020. Theranostics. 5.099305556. PubMed
RRID: AB_2563713 (BioLegend Cat. No. 362701) AB_2563714 (BioLegend Cat. No. 362702)
Structure: Type I membrane protein, part of the TCR-CD3 complex. 207 amino acids, predicted molecular weight of 23 kD.
Distribution: Mature T cells.
Function: Important role in T cell development and in linking antigen recognition with intracellular signaling pathways.
Interaction: Interacts with Nck.
Ligand/Receptor: MHC antigen.
Cell Type: T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Savage PD, et al. 1988. Cytogenet. Cell. Genet. 49:289. 2. DeJarnette JB, et al. 1998. Proc. Natl. Acad. Sci USA. 95:14909. 3. Wang B, et al. 1999. J. Immunol. 162:88. 4. Szymczak AL, et al. 2005. J. Immunol. 175:270. 5. Borroto A, et al. 2014. J. Immunol. 192:2042.
Gene ID: 916
UniProt: View information about CD3epsilon on UniProt.org
Clone: APA1/1
Regulatory Status: RUO
Other Names: CD3e molecule, epsilon (CD3-TCR complex), CD3e antigen, CD3 epsilon
Isotype: Mouse IgG1, κ