Biolegend, 364208, FITC anti-human CD222 (IGF2R) Recombinant Antibody, 100tests

QA19A18 recombinant antibody binds to CD222 in human, which is also known as the Insulin-like growth factor 2 receptor (IGF2R), cation-independent mannose-6-phosphate receptor (CIMPR), and mannose-6 phosphate receptor (M6PR). CD222 is a type I transmembrane glycoprotein present largely intracellularly in the Golgi apparatus and endosomal compartments, but also at the cell surface. CD222 is expressed ubiquitously in a vast majority of higher eukaryotic cell types and the central component of endosomal transport pathways. The variety of CD222 ligands implicates that CD222 plays important functions in many pathological processes, such as biogenesis of lysosomes, regulation of IGF2, LTGFβ activation, etc. Loss of CD222 is associated with inhibition of T effector cell function.
100tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality Tested FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2904390 (BioLegend Cat. No. 364207) AB_2904390 (BioLegend Cat. No. 364208)
Structure: Type I transmembrane glycoprotein
Distribution: Ubiquitously expressed on many cell types (Monocytes/Granulocytes)
Function: Biogenesis of lysosomes, embryogenesis, cell migration
Interaction: DPP4, heterotrimeric retromer cargo-selection complex (CSC)
Ligand/Receptor: Insulin-like growth factors, latent TGF-β, leukemia inhibitory factor (LIF), proliferin, prorenin, plasminogen
Molecular Family: Growth Factors
Antigen References: Leksa V, et al. 2017. Immunol Lett. 190:194. Pfisterer K, et al. 2014. J Immunol. 193:2718. Wood R, et al. 2008. J Biol Chem.
Gene ID: 3482
UniProt: View information about CD222 on UniProt.org
Clone: QA19A18
Regulatory Status: RUO
Other Names: CD222, Insulin-like growth factor 2 Receptor (IGF2R), Cation-independent mannose 6-phosphate receptor (CIMPR), or Mannose-6 phosphate receptor (M6PR)
Isotype: Mouse IgG1, κ