Biolegend, 365813, Brilliant Violet 421™ anti-human CD198 (CCR8) Antibody, 25tests

CCR8, also known as ChemR1, TER1, and CKR-L1, belongs to the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. CCR8 is expressed on some subsets of T cells, monocytes, macrophages, eosinophils, thymocytes, Langerhans cells, neurons, and vascular smooth muscle cells. Binding with its ligand CCL1, CCR8 plays a role in the regulation of monocyte chemotaxis and thymic cell apoptosis. It has been reported that CCR8 is also a receptor for CCL18. CCR8 is also believed to be an alternative coreceptor to CD4 for HIV-1 infection.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CCR8-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone S19016H induces complement-dependent cytotoxicity (CDC) and antibody-dependent cellular phagocytosis (ADCP) on CCR8-transfected cells.
RRID: AB_3097394 (BioLegend Cat. No. 365813) AB_3097394 (BioLegend Cat. No. 365814)
Structure: Seven hydrophobic transmembrane domains
Distribution: Vascular smooth muscle cells, macrophages, eosinophils, peritoneal macrophages, thymocytes, T cells, Langerhans cells and neurons
Cell Type: Tregs
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Tiffany HL, et al. 1997. J Exp Med. 186:165-70. Goya I, et al. 1998. J Immunol. 160:1975-81. Lee S, et al. 2000. J Virol. 74:6946-52. Chensue SW, et al. 2001. J Exp Med. 193:573-84. Qu C, et al. 2004. J Exp Med. 200:1231-41. Isolam SA, et al. 2013. J Exp Med. 210:1889-98.
Gene ID: 1237
UniProt: View information about CD198 on UniProt.org
Clone: S19016H
Regulatory Status: RUO
Other Names: CC-CKR-8, C-C CKR-8, CCR-8, CC chemokine receptor 8, CDw198, CDw198 antigen, CD198, chemokine (C-C motif) receptor 8, chemokine (C-C) receptor 8, TER1, ChemR1, and CKR-L1
Isotype: Rat IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.