Biolegend, 365824, PE/Dazzle™ 594 anti-human CD198 (CCR8) Antibody, 100tests

CCR8, also known as ChemR1, TER1, and CKR-L1, belongs to the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. CCR8 is expressed on some subsets of T cells, monocytes, macrophages, eosinophils, thymocytes, Langerhans cells, neurons, and vascular smooth muscle cells. Binding with its ligand CCL1, CCR8 plays a role in the regulation of monocyte chemotaxis and thymic cell apoptosis. It has been reported that CCR8 is also a receptor for CCL18. CCR8 is also believed to be an alternative coreceptor to CD4 for HIV-1 infection.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CCR8-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S19016H induces complement-dependent cytotoxicity (CDC) and antibody-dependent cellular phagocytosis (ADCP) on CCR8-transfected cells.
RRID: AB_3716977 (BioLegend Cat. No. 365823) AB_3716977 (BioLegend Cat. No. 365824)
Structure: Seven hydrophobic transmembrane domains
Distribution: Vascular smooth muscle cells, macrophages, eosinophils, peritoneal macrophages, thymocytes, T cells, Langerhans cells and neurons
Cell Type: Tregs
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Tiffany HL, et al. 1997. J Exp Med. 186:165-70. Goya I, et al. 1998. J Immunol. 160:1975-81. Lee S, et al. 2000. J Virol. 74:6946-52. Chensue SW, et al. 2001. J Exp Med. 193:573-84. Qu C, et al. 2004. J Exp Med. 200:1231-41. Isolam SA, et al. 2013. J Exp Med. 210:1889-98.
Gene ID: 1237
UniProt: View information about CD198 on UniProt.org
Clone: S19016H
Regulatory Status: RUO
Other Names: CC-CKR-8, C-C CKR-8, CCR-8, CC chemokine receptor 8, CDw198, CDw198 antigen, CD198, chemokine (C-C motif) receptor 8, chemokine (C-C) receptor 8, TER1, ChemR1, and CKR-L1
Isotype: Rat IgG2b, κ