Biolegend, 366505, PE anti-human CD257 (BAFF, BLYS) Antibody, 25tests

BAFF, also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog, activates apoptosis, NF-κB, and JNK (THANK). BAFF is a type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. It is upregulated by IFN-γ and downregulated by PMA/ionomycin treatment.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD257 transfected murine cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Hase H, et al. 2004. Blood 6:2257. (IHC)
RRID: AB_2565708 (BioLegend Cat. No. 366505) AB_2565709 (BioLegend Cat. No. 366506)
Structure: Type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. It can be proteolytically cleaved to form a soluble protein and it forms homotrimers. The predicted molecular weight of monomer is approximately 31 kD.
Distribution: Peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. Upregulated by IFN-γ and is downregulated by PMA/ionomycin treatment.
Function: Stimulates B and T cell immunity, regulates humoral immunity by binding TACI and BCMA receptors, and prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3).
Ligand/Receptor: BAFF binds to TACI (TNFRSF13B) and BCMA (TNFRSF17) as well as the APRIL ligand. BAFF also binds to the BAFF-specific receptor (BAFFR/BR3).
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
Molecular Family: CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
Antigen References: 1. Schneider P, et al. 1999. J. Exp. Med. 189:1747. 2. Moore PA, et al. 1999. Science 285:260. 3. Shu HB, et al. 1999. J. Leuko. Biol. 65:680. 4. Yu G, et al. 2000. Nat. Immunol. 1:252. 5. Oren DA, et al. 2002. Nat. Struct. Biol. 9:288.
Gene ID: 10673
UniProt: View information about CD257 on UniProt.org
Clone: 1D6
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: BAFF, BLYS, TNFSF13b, B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), TNF homolog that activates apoptosis, NF-κB and JNK (THANK)
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.