Biolegend, 366713, APC/Fire™ 750 anti-human IL-22 Antibody, 25tests

IL-22 is a cytokine that is structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF, also known as the IL-10-related T cell-derived inducible factor. IL-22 belongs to a family of cytokines with limited homology specifically to IL-10, IL-19, IL-20, IL-24, IL-26, the IFN-λs, IL-28A, IL-28B, and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Its activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor family. Two types of IL-22 binding receptors have been discovered: a membrane-bound receptor and a soluble receptor that are encoded by different genes. IL-22 is produced by Th17 cells and Th22 cells. The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant protein expressed in E. Coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2734410 (BioLegend Cat. No. 366713) AB_2734411 (BioLegend Cat. No. 366714)
Structure: Cytokine.
Distribution: Thymic lymphomas, T cells, activated T cells, macrophages, mast cells, and Th17.
Function: Enhances the production of acute phase proteins in HepG2 cells and promotes Th17 cells to trasmigrate through the blood-brain barrier in multiple sclerosis.
Interaction: IL-22 receptor.
Ligand/Receptor: IL-22R1 and IL-10R2 receptor chains.
Cell Type: Embryonic Stem Cells, Macrophages, Mast cells, T cells, Th17
Biology Area: Immunology, Stem Cells
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Nagalakshmi ML, et al. 2004. Int. Immunopharmacol. 5:679. 2. Kebir H,, et al. 2007. Nat. Med. 13:1173. 3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807. 4. Pene Y, et al. 2008. J. Immunol. 180:7423. 5. Dumotier L, et al. 2000. J. Immune 164:1814. 6. Xie MH, et al. 2000. J. Biol. Chem. 40:31335. 7. Kotenko SV, et al. 2001. J. Immunol. 166:7096. 8. Chung Y, et al. 2006. Cell Res. 11:902. 9. Trifari S, et al. 2009. Nat. Immunol. 10:864.
Gene ID: 50616
UniProt: View information about IL-22 on UniProt.org
Clone: 2G12A41
Regulatory Status: RUO
Other Names: IL22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18
Isotype: Mouse IgG2a, κ