Biolegend, 366903, PE anti-human IgG Fc Recombinant Antibody, 25tests

IgG Fc is a homodimer composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Anti-human IgG Fc antibody (clone QA19A42) may cross-react with bovine IgG. Anti-human IgG Fc antibody (clone M1310G05) does not exhibit this cross-reactivity.
Product Citations: Mulgaonkar A, et al. 2022. Clin Cancer Res. 28:4907. PubMed Fernandes LA, et al. 2022. Protein Sci. 31:e4355. PubMed
RRID: AB_2876689 (BioLegend Cat. No. 366903) AB_2876689 (BioLegend Cat. No. 366904)
Structure: Homodimer formed by the constant region of the IgG heavy chain
Ligand/Receptor: CD16, CD32, CD64
Cell Type: B cells, Basophils, Dendritic cells, Macrophages, Mast cells, Neutrophils, NK cells, Platelets
Biology Area: Adaptive Immunity, Cell Biology, Immunology, Innate Immunity
Molecular Family: Fc Receptors
Antigen References: Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.
Gene ID: 3500
UniProt: View information about IgG Fc on UniProt.org
Clone: QA19A42
Regulatory Status: RUO
Other Names: Immunoglobulin G
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.