Biolegend, 367105, Biotin anti-human CD14 Antibody, 25μg

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human peripheral blood monocytes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028. Devitt A, et al. 1998. Nature 6675:505.
Product Citations: Chen D, et al. 2020. J Exp Med. :217. PubMed Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
RRID: AB_2566617 (BioLegend Cat. No. 367105) AB_2566618 (BioLegend Cat. No. 367106)
Structure: GPI-linked membrane glycoprotein.
Distribution: Monocytes, macrophages, dendritic cells, and granulocytes.
Function: LPS receptor, clearance of Gram-negative pathogens.
Interaction: LPS.
Ligand/Receptor: LPS.
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Stocks SC, et al. 1990. Biochem. J. 268:275. Wright SD, et al. 1990. Science 4975:1431.
Gene ID: 929
UniProt: View information about CD14 on UniProt.org
Clone: 63D3
Regulatory Status: RUO
Other Names: Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.