Biolegend, 367715, APC anti-human KLRG1 (MAFA) Antibody, 25tests

Killer cell lectin-like receptor subfamily G member 1 (KLRG1) is a 30 kD, type II membrane glycoprotein with one C-type lectin domain and one immunoreceptor tyrosine-based inhibititory motif (ITIM). KLRG1 is expressed by subsets of NK, effector and memory T cells. KLRG1 inhibits cell activation and proliferation, and is a marker of T cell senescence. Binding of KLRG1 to E-, N-, or R- cadherins blocks phosphorylation of Akt and increases the expression of cell cycle inhibitors.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human KLRG1-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Gatla H, et al. 2022. Front Med Technol. 4:850565. PubMed Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
RRID: AB_2572160 (BioLegend Cat. No. 367715) AB_2572161 (BioLegend Cat. No. 367716)
Structure: Type II membrane glycoprotein, one C-type lectin domain, one ITIM, 30 kD.
Distribution: Subsets of NK, effector and memory T cells.
Function: Inhibits cell activation and proliferation, marker of T cell senescence.
Interaction: AKT
Ligand/Receptor: E-, N-, and R- cadherins
Cell Type: NK cells, T cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Shi L, et al. 2014. J. Immunol. 192:649.
Gene ID: 10219
UniProt: View information about KLRG1 on UniProt.org
Clone: SA231A2
Regulatory Status: RUO
Other Names: CLEC15A, MAFA-L, MAFA-2F1, MAFA-LIKE, 2F1
Isotype: Mouse IgG2a, κ