Biolegend, 368106, Alexa Fluor® 488 anti-human Sialyl Lewis X (dimeric) Antibody, 100tests

The FH6 antibody recognizes Sialyl Lewis X (demeric) on glycolipids or glycoproteins. It also recognizes Sialyl Lewis X with long carbohydrate attachments (Sialyl Lewis X-i). These antigens are expressed on human granulocytes, monocytes, small subsets of lymphocytes, some fetal tissues such as the fetal stomach, fetal colon, and fetal intestine, and a variety of cancer tissues. It is believed that these antigens are involved in cell adhesion.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified 6B fucoganglioside absorbed to Salmonella minnesota.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511. Fukushi Y, et al. 1985. Cancer Res. 8:3711. Kannagi R, et al. 1986. Cancer Research 5:2619. Kobayashi M, et al. 2010. Anticancer Res. 30:593.
RRID: AB_2566072 (BioLegend Cat. No. 368105)
Structure: Carbohydrate epitope; VI3NeuAcV3III3Fuc2nLc6.
Distribution: Granulocytes, monocytes, subpopulations of lymphocytes, fetal stomach, fetal colon, fetal small intestine, and a variety of cancer tissues.
Function: Cell adhesion.
Ligand/Receptor: Selectins.
Cell Type: Embryonic Stem Cells, Granulocytes, Lymphocytes, Monocytes, Neural Stem Cells, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511. 2. Kannagi R, et al. 1986. Cancer Research 5:2619. 3. Nakasaki H, et al. 1989. Cancer Research 49:3662. 4. Dohi T, et al. 1993. Anticancer Res. 13:1277.
Gene ID: NA
Clone: FH6
Regulatory Status: RUO
Other Names: CD15s, Stage-Specific Embryonic Antigen 1, SSEA-1, Sialyl Lewis X, sLex, sLe(x), Sialyl demeric LeX, Sialyl dermic Lewis X, Sialyl Lewis X-i, sLeX-i
Isotype: Mouse IgM, κ