Biolegend, 368539, Pacific Blue™ anti-human CD45 Antibody, 25tests

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBMC
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Application References(PubMed link indicates BioLegend citation): Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33. Csiba A, et al. 1984. Br. J. Cancer 50:699. Tchilian EZ, et al. 2001. J. Immunol. 166:1308. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations: Zhang Y, et al. 2023. Heliyon. 9:e14823. PubMed Frasca D, et al. 2023. PLoS One. 18:e0285025. PubMed Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed Wohlgemuth L, et al. 2022. Cells. 11: . PubMed Frasca D, et al. 2019. PLoS One. 14:e0219545. PubMed Kosaric N, et al. 2020. Mol Ther. 28:2007. PubMed Frasca D, et al. 2021. Immun Ageing. 18:35. PubMed Masuda T, et al. 2022. iScience. 25:104781. PubMed Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed Soto-Pantoja DR, et al. 2021. Cancer Res. 81:3890. PubMed Frasca D, et al. 2021. Int J Mol Sci. :22. PubMed Frasca D, et al. 2022. Front Aging. 2:828697. PubMed Frasca D, et al. 2021. Front Immunol. 616650:12. PubMed Frasca D, et al. 2018. PLoS One. 13:e0197472. PubMed Scharenberg SG, et al. 2020. Nat Commun. 2.76875. PubMed
RRID: AB_2716029 (BioLegend Cat. No. 368539) AB_2716030 (BioLegend Cat. No. 368540)
Structure: Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
Distribution: Hematopoietic cells, except circulating erythrocytes or platelets.
Function: Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Ligand/Receptor: Galectin-1, CD2, CD3, and CD4.
Cell Type: B cells, Dendritic cells, Neutrophils
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788
UniProt: View information about CD45 on UniProt.org
Clone: 2D1
Regulatory Status: RUO
Other Names: Leukocyte Common Antigen (LCA), T200
Isotype: Mouse IgG1, κ