Biolegend, 368608, PE/Dazzle™ 594 anti-human KLRG1 (MAFA) Antibody, 100tests

Killer cell lectin-like receptor subfamily G member (KLRG1) is a 30 kD, type II membrane glycoprotein with one C-type lectin domain and one immunoreceptor tyrosine-based inhibitory motif (ITIM). KLRG1 is expressed by subsets of natural killer cells (NKs) and effector and memory T cells. It inhibits cell activation and proliferation and is also a marker of T cell senescence. The binding of KLRG1 to E-, N-, or R- cadherins blocks phosphorylation of AKT and increases the expression of cell cycle inhibitors.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human KLRG1-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: van Montfoort N, et al. 2018. Cell. 175:1744. PubMed Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed
RRID: AB_2572134 (BioLegend Cat. No. 368607) AB_2572135 (BioLegend Cat. No. 368608)
Structure: Type II membrane glycoprotein, one C-type lectin domain, one ITIM, 30 kD.
Distribution: Subsets of natural killer cells, effector and memory T cells.
Function: Inhibits cell activation and proliferation
Interaction: AKT.
Ligand/Receptor: E-, N-, R- cadherins.
Cell Type: NK cells, T cells
Biology Area: Immunology
Antigen References: 1. Shi L, et al. 2014. J. Immunol. 192:649.
Gene ID: 10219
UniProt: View information about KLRG1 on UniProt.org
Clone: 14C2A07
Regulatory Status: RUO
Other Names: CLEC15A, MAFA-L, MAFA-2F1, MAFA-LIKE, 2F1
Isotype: Mouse IgG2a, κ