Biolegend, 368702, Purified anti-human Cadherin 11 Antibody, 100μg

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 11 is a type I transmembrane glycoprotein. It is a type II (atypical) cadherin, based on its lack of a HAV cell adhesion recognition sequence that is specific to type I cadherins. The expression of this particular cadherin in osteoblastic cell lines along with its upregulation during differentiation suggests a specific function in bone development and maintenance. This cadherin is also expressed in some cancer cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein containing the extracellular cadherin domain 1 of Cadherin 11 fused to maltose binding protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedICC, WB, IHC-P - VerifiedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/ml. For immunocytochemistry, a concentration rage of 2.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation.
Product Citations: Wei Z, et al. 2022. Cells. 11: . PubMed
RRID: AB_2566264 (BioLegend Cat. No. 368702)
Structure: Type I single pass transmembrane glycoprotein.
Distribution: Osteoblasts, neuroblasts, and some cancer cells.
Function: Cell adhesion.
Interaction: Cadherin 2 (CDH2).
Ligand/Receptor: Calcium.
Cell Type: Osteoclasts
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules
Antigen References: 1. Wheelock MJ, Johnson KR. 2003. Annu. Rev. Cell Dev. Biol. 19:207-35. 2. Christofori G, et al. 2003. EMBO. J. 22:2318. 3. Okazaki M, et al. 1994. J. Biol. Chem. 269:12092.
Gene ID: 1009
UniProt: View information about Cadherin 11 on UniProt.org
Clone: 16G5
Regulatory Status: RUO
Other Names: OSF-4, Osteoblast cadherin, OB-cadherin, CDH11, CDHOB, CAD11
Isotype: Mouse IgG1, κ